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Anti-Neurexin 3α (extracellular) Antibody

Nrxn3, NRX3A, Neurexin III-alpha

Cat #: ANR-033
Alternative Name Nrxn3, NRX3A, Neurexin III-alpha
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)RYLRWDASTRSDLS, corresponding to amino acid residues 39-52 of rat Nrxn3α (Accession Q07310). Extracellular, N-terminus.
Accession (Uniprot) Number Q07310
Gene ID 116508
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, human - identical.
RRID AB_2756688.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Specificity Designed to recognize Neurexin 3α and not Neurexin 3β.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.85 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ifc*, ip*, lci*
Western blot
  • Western blot analysis of mouse brain (lanes 1 and 5), rat brain (lanes 2 and 6), human brain neuroblastoma (SH-SY5Y) cells (lanes 3 and 7) and rat Pheochromocytoma (PC-12) cells (lanes 4 and 8):
    Western blot analysis of mouse brain (lanes 1 and 5), rat brain (lanes 2 and 6), human brain neuroblastoma (SH-SY5Y) cells (lanes 3 and 7) and rat Pheochromocytoma (PC-12) cells (lanes 4 and 8):
    1-4. Anti-Neurexin 3α (extracellular) Antibody (#ANR-033), (1:200).
    5-8. Anti-Neurexin 3α (extracellular) Antibody, preincubated with Neurexin 3α (extracellular) Blocking Peptide (#BLP-NR033).
Immunohistochemistry
  • Expression of Neurexin 3α in rat and mouse brain
    Expression of Neurexin 3α in rat and mouse brain
    Immunohistochemical staining of rat entorhinal cortex (A) and mouse striatum and lateral septum (B) using Anti-Neurexin 3α (extracellular) Antibody (#ANR-033), (1:400). A. Nrxn3α staining (green) appears in neuronal outlines in entorhinal cortex (arrow). B. Nrxn3α staining appeared in axonal processes in striatum (horizontal arrow) and in neuronal cell bodies in lateral septum (vertical arrows). DAPI is used as the counterstain (blue).
References
  1. Ichtchenko, K. et al. (1995) Cell 81, 435.
  2. Mosedale, M. et al. (2012) J. Biol. Chem. 287, 6350.
  3. Ullrich, B. et al. (1995) Neuron 14, 497.
  4. Zhang, C. et al. (2010) Neuron 66, 403.
  5. Craig, A.M. and Kang, Y. (2007) Curr. Opin. Neurobiol. 17, 43.
  6. Aoto, J. et al. (2013) Cell 154, 75.
Scientific background

Neurexins (NRXNs) are a family of transmembrane, synaptic adhesion molecules. NRXNS were identified as receptors for α-latrotoxin, a presynaptic toxin that triggers massive neurotransmitter release1. Neurexins are largely presynaptic proteins that form a trans-synaptic cell-adhesion complex with postsynaptic neuroligins2. They are encoded by three genes (NRXN1, NRXN2 and NRXN3), each using an upstream promoter to produce the longer α-isoform (α-NRXNs) and a downstream promoter to generate a shorter β-isoform (β-NRXNs).

The α-isoforms and β-isoforms of each neurexin are single-pass transmembrane proteins maintaining identical transmembrane and intracellular domains but having distinct extracellular domains. NRXNs in neurons localize to the presynaptic membrane and bind trans-synaptically to postsynaptic adhesion molecules and receptors3. Neurexins are expressed in all neurons, and are subject to extensive alternative splicing, generating >1,000 splice variants, some of which exhibit highly regulated developmental and spatial expression patterns4.

The structure of α-Neurexins is composed of 6 LNS domains (laminin, neurexin, sex-hormone-binding protein domain) and 3 EGF-like domains. In the mammalian brain they are known to interact with neuroligins, dystroglycan and neurexophilins. Neurexin 3α splicing can give rise to secreted and transmembrane forms of the protein. The biological function of the secreted form remains unknown5.

Changes in Neurexin 3α are associated with autism, drug addiction and obesity6.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 21/12/2020

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat Neurexin 3α. Anti-Neurexin 3α (extracellular) Antibody (#ANR-033) can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is therefore, highly suited to recognize Nrxn3α in living cells. It has been designed to recognize Nrxn3α from rat, mouse and human samples.

For research purposes only, not for human use
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