Overview
Type: Synthetic peptide
Form: Lyophilized powder
Neurofascin Blocking Peptide (#BLP-IP025) is the original antigen used for immunization during Anti-Neurofascin Antibody (#AIP-025) generation. The blocking peptide binds and ‘blocks’ Anti-Neurofascin primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) and rat hippocampus (lanes 3 and 6) lysates:1-3. Anti-Neurofascin Antibody (#AIP-025), (1:800).
4-6. Anti-Neurofascin Antibody, preincubated with Neurofascin Blocking Peptide (#BLP-IP025).
Expression of Neurofascin in rat spinal cord.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Neurofascin Antibody (#AIP-025), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Neurofascin immunoreactivity (green) appears dense in the dorsal horn (DH) and sparse in the dorsal column (DC). B. Pre-incubation of the antibody with Neurofascin Blocking Peptide (BLP-IP025), suppressed staining. Cell nuclei are stained with DAPI (blue).
Expression of Neurofascin in mouse hippocampus.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Neurofascin Antibody (#AIP-025), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the hippocampal dentate gyrus region, showed Neurofascin immunoreactivity (green) in the hilus (H) and in an outside layer (arrows). B. Pre-incubation of the antibody with Neurofascin Blocking Peptide (BLP-IP025), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)REKKDVPLGPEDPK, corresponding to amino acid residues 1142-1155 of rat NFASC (Accession P97685).
Accession (Uniprot) Number P97685
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
