Cat #: BLP-TR002
Type: Synthetic peptide
Form: Lyophilized powder
Neurokinin 2 Receptor/NK2R Blocking Peptide (#BLP-TR002) is the original antigen used for immunization during Anti-Neurokinin 2 Receptor (NK2R) Antibody (#ATR-002) generation. The blocking peptide binds and ‘blocks’ Anti-Neurokinin 2 Receptor/NK2R primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
WB-western blot, IHC-Immunohistochemistry
For research purposes only, not for human use
Demonstration of Pre-adsorption control
- Western blot analysis of rat hippocampus (lanes 1 and 3) and rat uterus (lanes 2 and 4):1,2. Anti-Neurokinin 2 Receptor (NK2R) Antibody (#ATR-002), (1:200).
3,4. Anti-Neurokinin 2 Receptor (NK2R) Antibody, preincubated with Neurokinin 2 Receptor/NK2R Blocking Peptide (#BLP-TR002).
Rat nodose ganglion sections (Yuan, M. et al. (2019) CNS Neurosci. Ther. 25, 123.).
- CHTKETLFMTGDMTHSE, corresponding to amino acid residues 351-367 of rat NK2 receptor (Accession P16610).
Accession (Uniprot) Number P16610
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.