Cat #: BLP-NR036
Type: Synthetic peptide
Form: Lyophilized powder
Neuroligin 2 (extracellular) Blocking Peptide (#BLP-NR036) is the original antigen used for immunization during Anti-Neuroligin 2 (extracellular) Antibody (#ANR-036) generation. The blocking peptide binds and ‘blocks’ Anti-Neuroligin 2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
WB-western blot, IHC-Immunohistochemistry
For research purposes only, not for human use
Demonstration of Pre-adsorption control
- Western blot analysis of rat brain membrane (lanes 1 and 4), mouse brain membrane (lanes 2 and 5) and rat PC12 pheochromocytoma cells (lanes 3 and 6):1-3. Anti-Neuroligin 2 (extracellular) Antibody (#ANR-036), (1:400).
4-6. Anti-Neuroligin 2 (extracellular) Antibody, preincubated with Neuroligin 2 (extracellular) Blocking Peptide (#BLP-NR036).
- Expression of NLGN2 in rat DRGImmunohistochemical staining of adult rat dorsal root ganglion (DRG) using Anti-Neuroligin 2 (extracellular) Antibody (#ANR-036) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. A. NLGN2 labeling (red) appears in the cell bodies of the DRG neurons. B. Nuclear staining using DAPI as the counter stain (blue). C. Merged image of A and B.
- Expression of NLGN2 in rat brainImmunnohistochemical staining of rat reticular thalamic nucleus using Anti-Neuroligin 2 (extracellular) Antibody (#ANR-036). A. NLGN2 staining (red) is detected in neuronal outlines (arrow). B. Nucleus staining using DAPI as the counterstain. C. Merged images of A and B.
- (C)DLGPRAYDRFPGDS, corresponding to amino acid residues 657-670 of rat NLGN2 (Accession Q62888).
Accession (Uniprot) Number Q62888
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.