Neuroplastin (extracellular) Blocking Peptide (#BLP-NR090) is the original antigen used for immunization during Anti-Neuroplastin (extracellular) Antibody (#ANR-090) generation. The blocking peptide binds and ‘blocks’ Anti-Neuroplastin (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain lysate (lanes 1 and 4), rat cerebellum lysate (lanes 2 and 5) and mouse brain membrane (lanes 3 and 6):
- Expression of Neuroplastin in rat hippocampus and rat cortexImmunohistochemical staining of free floating frozen section of rat hippocampal CA1 region (A) and posterior cingulate cortex (B) using Anti-Neuroplastin (extracellular) Antibody (#ANR-090), (1:120). A. NPTN staining (green) appears in neurons (vertical arrows) of the pyramidal layer (P) and in apical dendrites (horizontal arrows). B. NPTN staining (green) is detected in pyramidal neurons in the soma (vertical arrows) and in their apical dendrites (diagonal arrows). Nuclei were stained using DAPI as the counterstain (blue).
- (C)RKNASNMEYRINKPRAE, corresponding to amino acid residues 194-210 of rat NPTN (Accession P97546).