Neurotensin Receptor 2 Blocking Peptide (#BLP-NT016) is the original antigen used for immunization during Anti-Neurotensin Receptor 2 Antibody (#ANT-016) generation. The blocking peptide binds and ‘blocks’ Anti-Neurotensin Receptor 2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat dorsal root ganglion lysates:
- Western blot analysis of rat brain lysates:
- Western blot analysis of mouse brain lysates:
- Expression of NTR2 in rat mediobasal hypothalamusImmunohistochemical staining of perfusion-fixed brain frozen rat mediobasal (MB) hypothalamus sections using Anti-Neurotensin Receptor 2 Antibody (#ANT-016), (1:100), (green). A. NTR2 appears in neuronal fibers marked by white arrows. B. In the same section, there is staining for glial fibrillary acidic protein (red fluorescence), a marker of astrocytes. C. Merging the two images demonstrates that NTR2 is not localized in astrocytes. DAPI is used as the counterstain (blue).
- Expression of NTR2 in rat stomachImmunohistochemical staining of paraffin-embedded rat stomach sections using Anti-Neurotensin Receptor 2 Antibody (#ANT-016), (1:50), (brown). Staining is specific for parietal cells (arrows) of the gastric glands. Hematoxilin is used as the counterstain.
- CGEQHSLVPLPQEAPE, corresponding to amino acid residues 377-392 of rat NTSR2 (Accession Q63384).