Overview
Type: Synthetic peptide
Form: Lyophilized powder
Neurotensin Receptor 2 Blocking Peptide (#BLP-NT016) is the original antigen used for immunization during Anti-Neurotensin Receptor 2 Antibody (#ANT-016) generation. The blocking peptide binds and ‘blocks’ Anti-Neurotensin Receptor 2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat dorsal root ganglion lysates:1. Anti-Neurotensin Receptor 2 Antibody (#ANT-016), (1:200).
2. Anti-Neurotensin Receptor 2 Antibody, preincubated with Neurotensin Receptor 2 Blocking Peptide (#BLP-NT016).
Western blot analysis of rat brain lysates:1. Anti-Neurotensin Receptor 2 Antibody (#ANT-016), (1:200).
2. Anti-Neurotensin Receptor 2 Antibody, preincubated with Neurotensin Receptor 2 Blocking Peptide (#BLP-NT016).
Western blot analysis of mouse brain lysates:1. Anti-Neurotensin Receptor 2 Antibody (#ANT-016), (1:400).
2. Anti-Neurotensin Receptor 2 Antibody, preincubated with Neurotensin Receptor 2 Blocking Peptide (#BLP-NT016).
Expression of NTR2 in rat mediobasal hypothalamusImmunohistochemical staining of perfusion-fixed brain frozen rat mediobasal (MB) hypothalamus sections using Anti-Neurotensin Receptor 2 Antibody (#ANT-016), (1:100), (green). A. NTR2 appears in neuronal fibers marked by white arrows. B. In the same section, there is staining for glial fibrillary acidic protein (red fluorescence), a marker of astrocytes. C. Merging the two images demonstrates that NTR2 is not localized in astrocytes. DAPI is used as the counterstain (blue).
Expression of NTR2 in rat stomachImmunohistochemical staining of paraffin-embedded rat stomach sections using Anti-Neurotensin Receptor 2 Antibody (#ANT-016), (1:50), (brown). Staining is specific for parietal cells (arrows) of the gastric glands. Hematoxilin is used as the counterstain.
Properties
Sequence
- CGEQHSLVPLPQEAPE, corresponding to amino acid residues 377-392 of rat NTSR2 (Accession Q63384).
Accession (Uniprot) Number Q63384
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
