Neurotrophin 4/NT-4 Blocking Peptide (#BLP-NT004) is the original antigen used for immunization during Anti-Neurotrophin 4 (NT-4) Antibody (#ANT-004) generation. The blocking peptide binds and ‘blocks’ Anti-Neurotrophin 4/NT-4 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of Recombinant human Neurotrophin-4 (NT-4) protein (#N-270), (10 ng):
- Expression of Neurotrophin 4 in mouse spinal cordImmunohistochemical staining of mouse spinal cord using Anti-Neurotrophin 4 (NT-4) Antibody (#ANT-004). The spinal ventral horn (VH) was very lightly stained. However, in the perimeter of the white matter (WM) there were NT-4 immunoreactive cells with a small soma (triangles) and some had a stained process (arrows).
- LGEVPAAGGSPL(C), corresponding to amino acid residues 43-52 of mature human NT-4 (residues 123-132 of the precursor NT-4) (Accession P34130).