NGF Blocking Peptide (#BLP-N240) is the original antigen used for immunization during Anti-NGF Antibody (#AN-240) generation. The blocking peptide binds and ‘blocks’ Anti-NGF primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis using Anti-NGF Antibody (#AN-240) (1:200).
- Expression of NGF in rat brainImmunohistochemical staining of rat hippocampal mossy fiber terminal zone using Anti-NGF Antibody (#AN-240). A. The mossy fiber terminal zone (MF) is seen as the gray area (arrow) adjacent to the pyramidal layer (P). The pyramidal layer is visualized using cresyl violet (purple-labeled cells). B. The antibody was pre-incubated with the immunogen. The lack of staining in the MF zone (arrow) demonstrates specificity.
- Purified NGF 2.5S