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Anti-NGF Antibody

Nerve growth factor
Cat #: AN-240
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
    • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
    Immunogen
    Highly purified 2.5 S mouse NGF (Accession P01139).
    Accession (Uniprot) Number P01139
    Gene ID 18049
    Peptide confirmation Confirmed by DNA sequence and SDS-PAGE.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 5% sucrose (no preservative).
    Specificity The antibody does not react with BDNF, NT-3, and NT-4/5.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.3 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 μl PBS.
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1-3 μg antigen per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ih, wb
    May also work in: ic, ifc, ip
    Western blot
    Western blot analysis using Anti-NGF Antibody (#AN-240) (1:200).
    Immunohistochemistry
    Expression of NGF in rat brain
    Immunohistochemical staining of rat hippocampal mossy fiber terminal zone using Anti-NGF Antibody (#AN-240). A. The mossy fiber terminal zone (MF) is seen as the gray area (arrow) adjacent to the pyramidal layer (P). The pyramidal layer is visualized using cresyl violet (purple-labeled cells). B. The antibody was pre-incubated with the immunogen. The lack of staining in the MF zone (arrow) demonstrates specificity.
    References
    1. Roux, P. and Barker P.A. (2002) Prog. Neurobiol. 67, 203.
    2. Levi-Montalcini, R. (1966) Harvey Lect. 60, 217.
    3. Farinas, I. et al. (1998) Neuron 21, 325.
    4. Levi-Montalcini, R. et al. (1996) Trends Neurosci. 19, 514.
    5. Bradshaw, R.A. (1978) Ann. Rev. Biochem 47, 191.
    6. Bocchini, V. and Angeletti, P.U. (1969) Proc. Natl. Acad. Sci. U.S.A. 64, 787.
    7. McDonald, N.Q. et al. (1991) Nature 354, 411.
    8. Huang, E.J. and Reichardt, L.F. (2001) Annu. Rev. Neurosci. 24, 677.
    9. Freund, V. and Frossard, N. (1994) Prog. Brain Res. 146, 335.
    10. Raychaudhuri, S.P. and Raychaudhuri, S.K. (2004) Prog. Brain Res. 146, 433.
    11. Kawamoto, K. and Matsuda, H. (2004) Prog. Brain. Res. 146, 369.
    12. Teng, K.K. and Hempstead, B.L. (2004) Cell Mol. Life Sci. 61, 35.
    Scientific background

    The neurotrophins ("neuro" means nerve and "trophe" means nutrient) are a family of soluble, basic growth factors which regulate neuronal development, maintenance, survival and death in the CNS and the PNS.1 NGF, the first member of the family to be discovered, was originally purified as a factor able to support survival of sympathetic and sensory spinal neurons in culture.2 It is synthesized and secreted by sympathetic and sensory target organs and provides trophic support to neurons as they reach their final target.3 Neurotrophin secretion also increases in the nervous system following injury. Schwann cells, fibroblasts, and activated mast cells normally synthesize NGF constitutively, however direct trauma and induced cytokines combine to increase neurotrophin production in these cells after injury.4

    NGF is purified in three forms: the 7S, 2.5S and β. The 7S, 130 kDa, form occurs naturally in mouse submaxillary glands, and is a multimeric protein composed of two α, one β and two γ subunits. The name is derived from its sedimentation co-efficient, 7S. The biologically active subunit is the β, which is a 26 kDa dimer composed of two identical 120 amino acid chains held together by hydrophobic interactions.5 The 2.5S form is 9 amino acids shorter than the β form, because of proteolysis that occurs during the purification process.6 The structural hallmark of all the neurotrophins is the characteristic arrangement of the disulfide bridges known as the cysteine knot, which has been found in other growth factors such as PDGF.7 There is a 95.8% homology between the rat and mouse forms, and a 85% homology between the human and mouse.

    NGF has been shown to regulate neuronal survival, development function and plasticity.8 Recently, involvement of NGF in processes not involving neuronal cells has been shown, such as asthma,9 psoriasis10 and wound healing.11

    The biological effects of NGF are mediated by two receptors:
    TrkA, which is specific for NGF, and p75, which binds all the neurotrophins.12

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Lyophilized PowderControl Antigen Included
    receive a 25 µl free trial sample!
    Last update: 25/02/2019

    Alomone Labs is pleased to offer a highly specific antibody directed against mouse NGF. Anti-NGF Antibody (#AN-240) can be used in western blot and immunohistochemical applications. It has been designed to recognize NGF from mouse, rat and human samples. The antibody also recognizes proNGF.

    Related products for neutralizing NGF activity:
    Mouse NGF/proNGF Neutralizing Antibody (#ALM-006) can be used in indirect ELISA and neutralization assays to block the biological effects of both NGF and proNGF.

    For research purposes only, not for human use
    Citations
    Western blot citations
    1. Mouse retina lysate.
      Mysona, B.A. et al. (2013) Diabetologia 56, 2329.
    More product citations
    1. Matsuda, F. et al. (2011) Acta Physiol. 201, 275.
    2. Ali, T.K. et al. (2010) Diabetologia 54, 657.
    3. Lazo, O.M. et al. (2010) Mol. Neurodeg. 5, 5.
    4. Lecht, S. et al. (2010) Mol. Cell. Biochem. 339, 201.
    5. Schoenmann, Z. et al. (2010) J. Neurosci. 30, 6375.
    6. Takano, N. et al. (2007) Br. J. Dermatol. 156, 241.
    7. da Cruz, T.M. et al. (2005) Gene Ther. 12, 1242.
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