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- Roux, P. and Barker P.A. (2002) Prog. Neurobiol. 67, 203.
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The neurotrophins ("neuro" means nerve and "trophe" means nutrient) are a family of soluble, basic growth factors which regulate neuronal development, maintenance, survival and death in the CNS and the PNS.1 NGF, the first member of the family to be discovered, was originally purified as a factor able to support survival of sympathetic and sensory spinal neurons in culture.2 It is synthesized and secreted by sympathetic and sensory target organs and provides trophic support to neurons as they reach their final target.3 Neurotrophin secretion also increases in the nervous system following injury. Schwann cells, fibroblasts, and activated mast cells normally synthesize NGF constitutively, however direct trauma and induced cytokines combine to increase neurotrophin production in these cells after injury.4
NGF is purified in three forms: the 7S, 2.5S and β. The 7S, 130 kDa, form occurs naturally in mouse submaxillary glands, and is a multimeric protein composed of two α, one β and two γ subunits. The name is derived from its sedimentation co-efficient, 7S. The biologically active subunit is the β, which is a 26 kDa dimer composed of two identical 120 amino acid chains held together by hydrophobic interactions.5 The 2.5S form is 9 amino acids shorter than the β form, because of proteolysis that occurs during the purification process.6 The structural hallmark of all the neurotrophins is the characteristic arrangement of the disulfide bridges known as the cysteine knot, which has been found in other growth factors such as PDGF.7 There is a 95.8% homology between the rat and mouse forms, and a 85% homology between the human and mouse.
NGF has been shown to regulate neuronal survival, development function and plasticity.8 Recently, involvement of NGF in processes not involving neuronal cells has been shown, such as asthma,9 psoriasis10 and wound healing.11
Species reactivity key:
Alomone Labs is pleased to offer a highly specific antibody directed against mouse NGF. Anti-NGF Antibody (#AN-240) can be used in western blot and immunohistochemical applications. It has been designed to recognize NGF from mouse, rat and human samples. The antibody also recognizes proNGF.
Related products for neutralizing NGF activity:
Mouse NGF/proNGF Neutralizing Antibody (#ALM-006) can be used in indirect ELISA and neutralization assays to block the biological effects of both NGF and proNGF.
- Mouse retina lysate.
Mysona, B.A. et al. (2013) Diabetologia 56, 2329.
- Matsuda, F. et al. (2011) Acta Physiol. 201, 275.
- Ali, T.K. et al. (2010) Diabetologia 54, 657.
- Lazo, O.M. et al. (2010) Mol. Neurodeg. 5, 5.
- Lecht, S. et al. (2010) Mol. Cell. Biochem. 339, 201.
- Schoenmann, Z. et al. (2010) J. Neurosci. 30, 6375.
- Takano, N. et al. (2007) Br. J. Dermatol. 156, 241.
- da Cruz, T.M. et al. (2005) Gene Ther. 12, 1242.
- Recombinant human beta-NGF protein (#N-245)
- Native mouse NGF 2.5S protein (>95%) (#N-100)
- Native mouse NGF 2.5S protein (99%) (#N-240)
- Native mouse NGF 7S protein (#N-130)
- Recombinant human NGF proDomain protein (#N-290)
- Recombinant mouse proNGF protein (#N-250)
- Recombinant human proNGF (cleavage resistant) protein (#N-285)
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