Overview
Type: Synthetic peptide
Form: Lyophilized powder
Nicotinic Acetylcholine Receptor α4/CHRNA4 (extracellular) Blocking Peptide (#BLP-NC004) is the original antigen used for immunization during Anti-Nicotinic Acetylcholine Receptor α4 (CHRNA4) (extracellular) Antibody (#ANC-004) generation. The blocking peptide binds and ‘blocks’ Anti-Nicotinic Acetylcholine Receptor α4/CHRNA4 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain cell lysates:1,2. Anti-Nicotinic Acetylcholine Receptor α4 (CHRNA4) (extracellular) Antibody (#ANC-004), (1:200).
3,4. Anti-Nicotinic Acetylcholine Receptor α4 (CHRNA4) (extracellular) Antibody, preincubated with Nicotinic Acetylcholine Receptor α4/CHRNA4 (extracellular) Blocking Peptide (#BLP-NC004).
Expression of nAChR α4 in rat cerebellumImmunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-Nicotinic Acetylcholine Receptor α4 (CHRNA4) (extracellular) Antibody (#ANC-004), (1:200). A. Expression of nAChR α4 (green) was visualized in rat cerebellum. Purkinje cells were positive for nAChR α4 (arrows). B. The same section was stained for Parvalbumin (red) and counterstained (blue).
Multiplex staining of nAChR α4 and nAChR β2 in mouse brainImmunohistochemical staining of perfusion-fixed frozen mouse parietal cortex sections using Anti-Nicotinic Acetylcholine Receptor α4 (CHRNA4) (extracellular) Antibody (#ANC-004), (1:300) and Anti-Nicotinic Acetylcholine Receptor β2 (CHRNB2) (extracellular)-ATTO Fluor-594 Antibody (#ANC-012-AR), (1:60). A. nAChR α4 staining (green). B. Same section stained for nAChR β2 (red). C. Merge of the two images reveals several cells expressing both receptors (arrows). Nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)DLVNMHSRVDQLD, corresponding to amino acid residues 190-202 of human nAChRα4 (Accession P43681).
Accession (Uniprot) Number P43681
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
