NKCC2/SLC12A1 (extracellular) Blocking Peptide (#BLP-NT072) is the original antigen used for immunization during Anti-NKCC2 (SLC12A1) (extracellular) Antibody (#ANT-072) generation. The blocking peptide binds and ‘blocks’ Anti-NKCC2/SLC12A1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) kidney membranes:
- (C)GYKKNWRKAPLSELE, corresponding to amino acid residues 776-790 of rat NKCC2 (Accession P55016).