Cat #: BLP-GC020
Type: Synthetic peptide
Form: Lyophilized powder
A Blocking peptide for Anti-NMDAR2D (GRIN2D) (extracellular) Antibody (#AGC-020). Intended to be used as a negative control.
For research purposes only, not for human use
- Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes:
- Expression of NMDA receptor 2D in rat reticular thalamic nucleusImmunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-NMDAR2D (GRIN2D) (extracellular) Antibody (#AGC-020), (1:200). A. Staining (green) appears in a discreet population of cells (thin arrows). B. The same section was stained for parvalbumin (red) and cell nuclei were visualized with DAPI (blue). Cells positive for NR2D but negative for parvalbumin (thick arrow, shown in A) and cells positive for both NR2D and parvalbumin (vertical arrow) are both present.
- CRTQNRTHRGESLHR, corresponding to amino acid residues 345-359 of rat NMDAR2D (Accession Q62645).
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
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