Overview
Type: Synthetic peptide
Form: Lyophilized powder
NMDAR3B/GRIN3B (extracellular) Blocking Peptide (#BLP-GC031) is the original antigen used for immunization during Anti-NMDAR3B (GRIN3B) (extracellular) Antibody (#AGC-031) generation. The blocking peptide binds and ‘blocks’ Anti-NMDAR3B/GRIN3B (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:1,2. Anti-NMDAR3B (GRIN3B) (extracellular) Antibody (#AGC-031), (1:200).
3,4. Anti-NMDAR3B (GRIN3B) (extracellular) Antibody, preincubated with NMDAR3B/GRIN3B (extracellular) Blocking Peptide (#BLP-GC031).
Expression of NR3B in mouse neocortexImmunohistochemical staining of immersion-fixed, free floating mouse brain frozen sections using Anti-NMDAR3B (GRIN3B) (extracellular) Antibody (#AGC-031), (1:100). NR3B expression (red) is most striking in pyramidal neurons (arrows). DAPI staining of cell nuclei (blue) was used as a general cellular marker.
Multiplex staining of NMDA3B and NMDAR1 in mouse cortex.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-NMDAR3B (GRIN3B) (extracellular) Antibody (#AGC-031), (1:400), followed goat anti-rabbit-AlexaFluor-488 and Guinea pig Anti-NMDAR1 (GluN1) (extracellular) Antibody (#AGC-001-GP), (1:1200), followed by goat anti-donkey-biotin and streptavidin-Cy3. A. NMDA3A immunoreactivity (green) appears in pyramidal cells of layer 5. B. NMDAR1 immunoreactivity (red) appears in several cell types in the same region. C. Merge of the two images reveals several cells expressing both NMDA3B and NMDAR1 (arrows point at examples). Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)HVSRHFKVWSLRRD, corresponding to amino acid residues 363-376 of rat NMDAR3B (Accession Q8VHN2).
Accession (Uniprot) Number Q8VHN2
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
