NMUR2 (extracellular) Blocking Peptide (#BLP-NR072) is the original antigen used for immunization during Anti-NMUR2 (extracellular) Antibody (#ANR-072) generation. The blocking peptide binds and ‘blocks’ Anti-NMUR2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain (lanes 1 and 5), mouse brain (lanes 2 and 6), human brain glioblastoma (U-87 MG) (lanes 3 and 7) and mouse brain glioma (C6) cell (lanes 4 and 8) lysates:
- Expression of Neuromedin U Receptor 2 in rat hippocampus.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-NMUR2 (extracellular) Antibody (#ANR-072), (1:100), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the rat hippocampal CA1 region, showed immunoreactivity (green) in astrocytes (horizontal arrows), and neurons (vertical arrows). B. Pre-incubation of the antibody with NMUR2 (extracellular) Blocking Peptide (BLP-NR072), suppressed staining. Cell nuclei are stained with DAPI (blue).
- (C)KLENASWIHDPLMK, corresponding to amino acid residues 3-16 of rat NMUR2 (Accession Q9ESQ4).