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NNC 55-0396 dihydrochloride

A Blocker of T-type CaV Channels

Cat #: N-205
Lyophilized Powder yes
  • Bioassay Tested
  • Source Synthetic
    MW: 564.57
    Purity: >95%
    Form Lyophilized powder.
    Effective concentration 1-100 µM.
    Structure
    Chemical name (1S,2S)-2-[2-[[3-(1H-Benzimidazol-2­-yl)propyl]methylamino]ethyl]-6-fluoro-1,2,3,4-tet­rahydro-1-(1-methylethyl)-2-naphthalenyl cyclopropanecarboxylate dihydrochloride.
    Molecular formula C30H39.8Cl1.8FN3O2.
    CAS No.: 357400-13-6
    Activity NNC 55-0396 displays IC50 values of 6.8 and >100 μM for inhibition of CaV3.1 T-type channels and HVA currents respectively in INS-1 cells1.
    References-Activity
    1. Huang, L. et al. (2004) J. Pharmacol. Exp. Ther309, 193.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility 50 mM in water. Centrifuge all product preparations before use (10000 x g for 1 min).
    Storage of solutions Up to four weeks at 4°C or three months at -20°C.
    Our bioassay
    • Alomone Labs NNC 55-0396 dihydrochloride inhibits CaV3.2 channels heterologously expressed in Xenopus oocytes.
      Alomone Labs NNC 55-0396 dihydrochloride inhibits CaV3.2 channels heterologously expressed in Xenopus oocytes.
      A. Time course of CaV3.2 peak current amplitude, elicited by 100 ms voltage step from holding potential of -100 mV to -20 mV, delivered every 10 seconds. Application of 10 µM NNC 55-0396 dihydrochloride (#N-205) inhibits the CaV3.2 current in a reversible manner (indicated by the horizontal bar). B. Representative current traces before and during application of 10 µM NNC 55-0396 dihydrochloride, as indicated.
    References - Scientific background
    1. Huang, L. et al. (2004) J. Pharmacol. Exp. Ther309, 193.
    2. Chen, W.K. et al. (2010) J. Neurosci30, 10360.
    Scientific background NNC 55-0396 dihydrochloride, is a structural analog of mibefradil (Ro 40-5967) that inhibits both T-type and high-voltage-activated (HVA) Ca2+ channels with a higher selectivity for T-type Ca2+ channels. NNC 55-0396 exerts no effect against high voltage Ca2+ channels at 100 μM, but inhibits T-type channels in HEK293 cells with a potency comparable to that of mibefradil (IC50 values of 6.8 versus 10.1 μM)1. NNC 55- 0396 can be an essential tool in preventing human ovarian cancer cell proliferation as a result of its ability to inhibit the function of T-type Ca2+ channels. It is believed that NNC 55-0396 may function by dissolving in or passing through the plasma membrane of cells2.
    Target T-type CaV channels
    Last update: 24/12/2020

    NNC 55-0396 dihydrochloride (#N-205) is a highly pure, synthetic, and biologically active compound.

    For research purposes only, not for human use
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