Nogo Receptor (extracellular) Blocking Peptide (#BLP-NT008) is the original antigen used for immunization during Anti-Nogo Receptor (extracellular) Antibody (#ANT-008) generation. The blocking peptide binds and ‘blocks’ Anti-Nogo Receptor (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain lysate:
- Expression of Nogo receptor in rat cerebellumImmunohistochemical staining of rat cerebellum using Anti-Nogo Receptor (extracellular) Antibody (#ANT-008). A. NgR (red) appears in Purkinje cells (arrows). B. Staining of Purkinje nerve cells with mouse anti-calbindin D28K (a calcium binding protein, green). C. Confocal merge of NgR and calbindin D28K demonstrates some co-localization of these proteins.
- (C)GDSPPGNGSGPRHIND corresponding to amino acid residues 366-381 of the human Nogo receptor (Accession Q9BZR6).