Cat #: BLP-MT002
Type: Synthetic peptide
Form: Lyophilized powder
A Blocking peptide for Anti-Noradrenaline Transporter (NET) (extracellular) Antibody (#AMT-002). Intended to be used as a negative control.
For research purposes only, not for human use
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:1,2. Anti-Noradrenaline Transporter (NET) (extracellular) Antibody (#AMT-002), (1:400).
3,4. Anti-Noradrenaline Transporter (NET) (extracellular) Antibody, preincubated with Noradrenaline Transporter/NET (extracellular) Blocking Peptide (#BLP-MT002).
- Expression of Noradrenaline transporter (NET) in rat hippocampal dentate gyrusImmunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-Noradrenaline Transporter (NET) (extracellular) Antibody (#AMT-002), (1:200). A. NET (green) is visualized in axonal processes (arrows) in the hilus. B. The relation to layers was explored using mouse anti-GAP43 (red). There are less axonal processes in the outer molecular layer (asterisk) compared to the inner molecular layer (arrows). C. Overlay of A and B shows co-localization of NET and GAP43 in the outer molecular layer.
- (C)KLLNASVLGDHTKYSK, corresponding to amino acid residues 189-204 of mouse NET (Accession O55192).
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
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