Overview
Type: Synthetic peptide
Form: Lyophilized powder
Noradrenaline Transporter/NET (extracellular) Blocking Peptide (#BLP-MT002) is the original antigen used for immunization during Anti-Noradrenaline Transporter (NET) (extracellular) Antibody (#AMT-002) generation. The blocking peptide binds and ‘blocks’ Anti-Noradrenaline Transporter/NET (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:1,2. Anti-Noradrenaline Transporter (NET) (extracellular) Antibody (#AMT-002), (1:400).
3,4. Anti-Noradrenaline Transporter (NET) (extracellular) Antibody, preincubated with Noradrenaline Transporter/NET (extracellular) Blocking Peptide (#BLP-MT002).
Expression of Noradrenaline transporter (NET) in rat hippocampal dentate gyrusImmunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-Noradrenaline Transporter (NET) (extracellular) Antibody (#AMT-002), (1:200). A. NET (green) is visualized in axonal processes (arrows) in the hilus. B. The relation to layers was explored using mouse anti-GAP43 (red). There are less axonal processes in the outer molecular layer (asterisk) compared to the inner molecular layer (arrows). C. Overlay of A and B shows co-localization of NET and GAP43 in the outer molecular layer.
Properties
Sequence
- (C)KLLNASVLGDHTKYSK, corresponding to amino acid residues 189-204 of mouse NET (Accession O55192).
Accession (Uniprot) Number O55192
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
