Overview
Type: Synthetic peptide
Form: Lyophilized powder
Nucleobindin-2/Nesfatin-1 Blocking Peptide (#BLP-CS011) is the original antigen used for immunization during Anti-Nucleobindin-2/Nesfatin-1 Antibody (#ACS-011) generation. The blocking peptide binds and ‘blocks’ Anti-Nucleobindin-2/Nesfatin-1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain (lanes 1 and 5), mouse brain (lanes 2 and 6), rat pancreas (lanes 3 and 7) and rat pituitary (lanes 4 and 8):1,2,4. Anti-Nucleobindin-2/Nesfatin-1 Antibody (#ACS-011), (1:200).
3. Anti-Nucleobindin-2/Nesfatin-1 Antibody (1:400).
5-8. Anti-Nucleobindin-2/Nesfatin-1 Antibody, preincubated with Nucleobindin-2/Nesfatin-1 Blocking Peptide (#BLP-CS011).
Western blot analysis of human recombinant Nucleobindin-2/Nesfatin-1 (200 ng):1. Anti-Nucleobindin-2/Nesfatin-1 Antibody (#ACS-011) (1:200).
2. Anti-Nucleobindin-2/Nesfatin-1 Antibody, preincubated with Nucleobindin-2/Nesfatin-1 Blocking Peptide (#BLP-CS011).
Expression of Nucleobindin-2/Nesfatin-1 in rat hypothalmus.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Nucleobindin-2/Nesfatin-1 Antibody (#ACS-011), (1:1200), followed by goat anti-rabbit-Alexa-488. A. Staining in the ventromedial hypothalamus (VMH) region showed Nucleobindin-2/Nesfatin-1 immunoreactivity (green) in VMH neurons (arrows). B. Pre-incubation of the antibody with Nucleobindin-2/Nesfatin-1 Blocking Peptide (#BLP-CS011), suppressed staining. Cell nuclei are stained with DAPI (blue). 3rd V = Third ventricle
Properties
Sequence
- (C)DKTKVHNVEPVESAR, corresponding to amino acid residues 30-44 of rat Nucleobindin-2/Nesfatin-1 (Accession Q9JI85).
Accession (Uniprot) Number Q9JI85
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
