Overview
V36 - Valine amide.
- Lampe R.A. et al. (1993) Mol. Pharmacol. 44, 451.
Soluble in water. For long-term storage in solution, we recommend to prepare a stock solution by dissolving the product in ddH2O at a concentration X100-1000 of final working solution. Divide the solution into small aliquots and store at -20°C. Upon use, thaw the relevant vial intended for use and dilute in your desired working buffer. Centrifuge all product preparations before use (10000 x g, 5 min). Repeat freeze-thawing might result in loss of activity. [It is recommended to prepare working solutions fresh before use].
Alomone Labs ω-Grammotoxin SIA inhibits CaV2.2 channel currents heterologously expressed in Xenopus oocytes.A. Representative time course of ω-Grammotoxin SIA (#STG-450) inhibition of CaV2.2 (α1B + α2δ1 + β1) channels current. Membrane potential was held at -100 mV, current was elicited by a 100 ms voltage ramp to +60 mV every 10 sec, and inhibited by 500 nM ω-Grammotoxin SIA (green).
B. Superimposed traces of CaV2.2 channel currents in the absence (control) and presence (green) of 500 nM ω-Grammotoxin SIA (taken from the recording in A).
- Lampe R.A. et al. (1993) Mol. Pharmacol. 44, 451.
- Piser T.M. et al. (1995) Mol. Pharmacol. 48, 131.
- McDonough, S.I. et al. (1997) Mol. Pharmacol. 52, 1095.
ω-Grammotoxin SIA is a 36 amino acid peptidyl toxin originally isolated from the venom of the South American Tarantula spider, Grammostola spatulata. ω-Grammotoxin SIA potently inhibits both CaV2.1 (P-type) and CaV2.2 (N-type) Ca2+ channels by altering the voltage-dependence of channel gating. ω-Grammotoxin SIA caused a concentration-dependent and virtually complete inhibition of K+-evoked influx of 45Ca2+ into either rat or chick brain synaptosomes.1 ω-Grammotoxin SIA at 1 µM, a maximally effective concentration, blocked 52% of IBa in cultured rat hippocampal neurons.2 A concentration of >50 nM toxin completely inhibited Ca2+ currents in Purkinje neurons (predominantly, P-type channels) and in sympathetic neurons (predominantly, N-type channels).3
ω- Grammotoxin SIA (#STG-450) is a highly pure, synthetic, and biologically active peptide toxin.
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