Overview
Type: Synthetic peptide
Form: Lyophilized powder
OXGR1/GPR99 Blocking Peptide (#BLP-OR016) is the original antigen used for immunization during Anti-OXGR1/GPR99 Antibody (#AOR-016) generation. The blocking peptide binds and ‘blocks’ Anti-OXGR1/GPR99 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse kidney (lanes 1 and 4), rat kidney (lanes 2 and 5) (1:600) and rat testis (lanes 3 and 6) (1:200) lysates:1-3. Anti-OXGR1/GPR99 Antibody (#AOR-016).
4-6. Anti-OXGR1/GPR99 Antibody, preincubated with OXGR1/GPR99 Blocking Peptide (#BLP-OR016).
Expression of OXGR1/GPR99 in mouse hippocampus and cerebellumImmunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-OXGR1/GPR99 Antibody (#AOR-016), (1: 200), followed by goat anti-rabbit-AlexaFluor-488. A. GPR99 staining (green) in hippocampal dentate gyrus, is detected in the granule layer (G, vertical arrows) and in neurons of the hilus (H, horizontal arrows). B. GPR99 immunoreactivity (green) in cerebellum, is observed in neurons of the purkinje cell layer (P, vertical arrows). Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- CKASGDLEQGKKDS, corresponding to amino acid residues 319-332 of mouse OXGR1 (Accession Q6IYF8).
Accession (Uniprot) Number Q6IYF8
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
