OXGR1/GPR99 Blocking Peptide (#BLP-OR016) is the original antigen used for immunization during Anti-OXGR1/GPR99 Antibody (#AOR-016) generation. The blocking peptide binds and ‘blocks’ Anti-OXGR1/GPR99 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of mouse kidney (lanes 1 and 4), rat kidney (lanes 2 and 5) (1:600) and rat testis (lanes 3 and 6) (1:200) lysates:
- Expression of OXGR1/GPR99 in mouse hippocampus and cerebellumImmunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-OXGR1/GPR99 Antibody (#AOR-016), (1: 200), followed by goat anti-rabbit-AlexaFluor-488. A. GPR99 staining (green) in hippocampal dentate gyrus, is detected in the granule layer (G, vertical arrows) and in neurons of the hilus (H, horizontal arrows). B. GPR99 immunoreactivity (green) in cerebellum, is observed in neurons of the purkinje cell layer (P, vertical arrows). Cell nuclei are stained with DAPI (blue).
- CKASGDLEQGKKDS, corresponding to amino acid residues 319-332 of mouse OXGR1 (Accession Q6IYF8).