P2X1 Receptor Blocking Peptide (#BLP-PR001) is the original antigen used for immunization during Anti-P2X1 Receptor Antibody (#APR-001) generation. The blocking peptide binds and ‘blocks’ Anti-P2X1 Receptor primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of human platelet lysates:
- Expression of P2X1 Receptor in rat hypothalamusImmunohistochemical staining of rat supra-optic nucleus using Anti-P2X1 Receptor Antibody (#APR-001). P2X1 receptor (green) appears in axonal processes (vertical arrows) and cell bodies (horizontal arrows). DAPI is used as the counterstain (blue).
- Mouse mesenteric arteries (Vial, C. and Evans, R.J. (2002) Mol. Pharmacol. 62, 1438.).
- (CS)DPVATSSTLGLQENMRTS, corresponding to amino acid residues 382-399 of rat P2X1 receptor (Accession P47824).