Cat #: BLP-PR024
Type: Synthetic peptide
Form: Lyophilized powder
P2X4 Receptor (extracellular) Blocking Peptide (#BLP-PR024) is the original antigen used for immunization during Anti-P2X4 Receptor (extracellular) Antibody (#APR-024) generation. The blocking peptide binds and ‘blocks’ Anti-P2X4 Receptor (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
WB-western blot, IHC-Immunohistochemistry
For research purposes only, not for human use
Demonstration of Pre-adsorption control
- Western blot analysis of rat brain membrane (lanes 1 and 5), mouse brain membrane (lanes 2 and 6), C6 rat brain glioma cell line lysate (lanes 3 and 7) and SH-SY5Y human brain neuroblastoma cell line lysate (lanes 4 and 8):1-4. Anti-P2X4 Receptor (extracellular) Antibody (#APR-024), (1:200).
5-8. Anti-P2X4 Receptor (extracellular) Antibody, preincubated with P2X4 Receptor (extracellular) Blocking Peptide (#BLP-PR024).
- (C)RDLAGKEQRTLTK, corresponding to amino acid residues 301-313 of rat P2X4 receptor (Accession P51577).
Accession (Uniprot) Number P51577
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.