P2X7 Receptor Blocking Peptide (#BLP-PR004) is the original antigen used for immunization during Anti-P2X7 Receptor Antibody (#APR-004) generation. The blocking peptide binds and ‘blocks’ Anti-P2X7 Receptor primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat hippocampus lysates:
- Expression of P2X7 Receptor in rat pancreasImmunohistochemical staining of rat paraffin-embedded endocrine and exocrine pancreas sections using Anti-P2X7 Receptor Antibody (#APR-004), (1:50), followed by goat anti-rabbit-AlexaFluor-555-secondary antibody (1:500), (red). Staining is highly specific for endocrine cells of the Isle of Langerhans. Hoechst 33342 is used as the counterstain (blue).
- (C)KIRKEFPKTQGQYSGFKYPY, corresponding to amino acid residues 576-595 of rat P2X7 receptor (Accession Q64663).