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Certificate of Analysis

P2Y Receptor Antibody Explorer Kit

A Screening Package of P2Y Receptor Antibodies Economically Priced

Cat #: AK-100
Size: 22 Vials
Form: Lyophilized

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Anti-P2Y1 Receptor Antibody Cat #: APR-009

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, IP, WB Reactivity: H, M, R
Immunogen
Peptide (C)RALI YKDLD NSPLR RKS, corresponding to residues 242-258 of rat or human P2RY1. (Accession P49651P47900). 3rd intracellular loop (i3) between TM5 and TM6 domains.
Homology
Rat, human, bovine - identical; turkey, chicken - 16/17 amino acid residues identical; mouse - 15/17 amino acid residues identical.

P2Y1 Receptor Blocking Peptide Cat #: BLP-PR009

Size: 40 µg

Anti-P2Y1 Receptor (extracellular) Antibody Cat #: APR-021

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, LCI, WB Reactivity: H, M, R
Immunogen
Peptide SDEYLRSYFIYSMC, corresponding to amino acid residues 207-220 of human P2RY1 (Accession P47900). 2nd extracellular loop.
Homology
Rat - identical; mouse - 12/14 amino acid residues identical.

P2Y1 Receptor (extracellular) Blocking Peptide Cat #: BLP-PR021

Size: 40 µg

Anti-P2Y2 Receptor Antibody Cat #: APR-010

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, IP, WB Reactivity: H, M, R
Immunogen
Peptide (C)KPAY GTTGL PRAKR KSVR, corresponding to residues 227-244 of rat P2RY2 (Accession P41232). 3rd intracellular loop (i3) between TM5 and TM6 domains.
Homology
Mouse - 18/19 amino acid residues identical; human - 17/19 amino acid residues identical.

P2Y2 Receptor Blocking Peptide Cat #: BLP-PR010

Size: 40 µg

Anti-P2Y2 Receptor (extracellular) Antibody Cat #: APR-102

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, LCI, WB Reactivity: H, M, R
Immunogen
Peptide (CD)TINGTWEGDELGYK, corresponding to amino acid residues 11- 24 of rat P2RY2 (Accession P41232). Extracellular, C-terminus.
Homology
Rat, mouse – 14/15 amino acid residues identical; human – 13/15 amino acid residues identical.

P2Y2 Receptor (extracellular) Blocking Peptide Cat #: BLP-PR102

Size: 40 µg

Anti-P2Y4 Receptor Antibody Cat #: APR-006

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, IP, WB Reactivity: H, M, R
Immunogen
Peptide (C)HEES ISRWA DTHQD, corresponding to residues 337-350 of rat P2RY4 (Accession O35811). Intracellular, C-terminus.
Homology
Mouse - 13/14 amino acid residues identical.

P2Y4 Receptor Blocking Peptide Cat #: BLP-PR006

Size: 40 µg

Anti-P2Y6 Receptor Antibody Cat #: APR-011

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, IP, WB Reactivity: H, M, R
Immunogen
Peptide CQPHDLLQKLTAKWQRQRV, corresponding to amino acid residues 311-328 of rat P2Y6 receptor (Accession Q63371). Intracellular, C-terminus.
Homology
Mouse - 17/18 amino acid residues identical; human - 15/18 amino acid residues identical.

P2Y6 Receptor Blocking Peptide Cat #: BLP-PR011

Size: 40 µg

Anti-Human P2Y11 Receptor Antibody Cat #: APR-015

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IP, WB Reactivity: H
Immunogen
Peptide (C)NATAAPKPSEPQSRELS, corresponding to amino acid residues 357-373 of human P2RY11 (Accession Q96G91). Intracellular, C-terminus.
Homology
Human only. Not recommended for use with rat and mouse samples.

Human P2Y11 Receptor Blocking Peptide Cat #: BLP-PR015

Size: 40 µg

Anti-P2Y12 Receptor Antibody Cat #: APR-012

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, IP, WB Reactivity: H, M, R
Immunogen
Peptide (C)KTTRPFKTSNPKNLLGAK,  corresponding to amino acid residues 125-142 of human P2RY12 (Accession Q9H244). Intracellular, 2nd cytoplasmic loop.
Homology
Rat, mouse - 16/18 amino acid residues identical.

P2Y12 Receptor Blocking Peptide Cat #: BLP-PR012

Size: 40 µg

Anti-P2Y12 Receptor (extracellular) Antibody Cat #: APR-020

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, LCI, WB Reactivity: H, R
Immunogen
Peptide CTAENTLFYVKES, corresponding to amino acid residues 270-282 of human P2RY12 (Accession Q9H244). 3rd extracellular loop.
Homology
Rat, mouse - 12/13 amino acid residues identical.

P2Y12 Receptor (extracellular) Blocking Peptide Cat #: BLP-PR020

Size: 40 µg

Anti-P2Y13 Receptor Antibody Cat #: APR-017

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, WB Reactivity: H, M, R
Immunogen
Peptide DRFLKIIRPLRNIFLK(C), corresponding to amino acid residues 119-134 of human P2RY13 (Accession Q9BPV8). 2nd intracellular loop.
Homology
Mouse - 11/16 amino acid residues identical; rat - 10/16 amino acid residues identical.

P2Y13 Receptor Blocking Peptide Cat #: BLP-PR017

Size: 40 µg

Anti-P2Y14 Receptor (extracellular) Antibody Cat #: APR-018

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IF, IFC, IH, LCI, WB Reactivity: H, M, R
Immunogen
Peptide CIELKSELGRKWHKASN, corresponding to amino acid residues 172-188 of human P2RY14 (Accession Q15391). 2nd extracellular loop.
Homology
Rat, mouse - 15/17 amino acid residues identical.

P2Y14 Receptor (extracellular) Blocking Peptide Cat #: BLP-PR018

Size: 40 µg

Antibody Storage & Reconstitution Instructions

Storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Add deionized water, depending on the sample size.

Storage after reconstitution

The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).

Control antigen storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Control antigen reconstitution

100 μl water.

Control antigen storage after reconstitution

-20ºC.

Preadsorption control

1 μg peptide per 1 μg antibody.

For research purposes only, not for human use
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