P2Y6 Receptor (extracellular) Blocking Peptide (#BLP-PR106) is the original antigen used for immunization during Anti-P2Y6 Receptor (extracellular) Antibody (#APR-106) generation. The blocking peptide binds and ‘blocks’ Anti-P2Y6 Receptor (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) heart membranes:
- Western blot analysis of mouse BV-2 microglia cell line lysate (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):
- Western blot analysis of human MDA-MB-231 breast adenocarcinoma cell line lysate:
- Expression of P2RY6 in rat parietal cortexImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-P2Y6 Receptor (extracellular) Antibody (#APR-106), (1:1000), followed by donkey-anti-rabbit-biotin and streptavidin-Cy3. P2RY6 immunoreactivity (red) appears in pyramidal neurons (arrows). Cell nuclei are stained with DAPI (blue).
- (C)RNRTV(S)YDLSPPILS, corresponding to amino acid residues 172 - 186 of mouse P2RY6 (Accession Q9ERK9).