PACSIN3 Blocking Peptide (#BLP-IP015) is the original antigen used for immunization during Anti-PACSIN3 Antibody (#AIP-015) generation. The blocking peptide binds and ‘blocks’ Anti-PACSIN3 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain membrane (lanes 1 and 5), rat skeletal muscle lysate (lanes 2 and 6), rat heart lysate (lanes 3 and 7) and mouse heart membrane (lanes 4 and 8):
- (C)RKDEKTAQTRESHAK, corresponding to amino acid residues 161-175 of mouse Pacsin3 (Accession Q99JB8).