Pannexin 1 Blocking Peptide (#BLP-CC234) is the original antigen used for immunization during Anti-Pannexin 1 Antibody (#ACC-234) generation. The blocking peptide binds and ‘blocks’ Anti-Pannexin 1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of mouse (lanes 1 and 5) and rat (lanes 2 and 6) heart lysates and mouse (lanes 3 and 7) and rat (lanes 4 and 8) brain membranes:
- Expression of Pannexin 1 in rat cortexImmunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-Pannexin 1 Antibody (#ACC-234), (1:300). PANX1 staining (green) is apparent in cells with neuronal outline (vertical arrows) and apical dendrites (horizontal arrows) in the parietal cingulated cortex region. Cell nuclei are stained with DAPI (blue).
- (C)KEPTEPKFKGLRLE, corresponding to amino acid residues 18- 31 of human PANX1 (Accession Q96RD7).