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PD 98059

A Potent Inhibitor of Mitogen-Activated Protein Kinase (MAPK) Pathway
Cat #: P-260
Lyophilized Powder yes
  • Bioassay Tested
  • Source Synthetic
    MW: 267.28
    Purity: >99%
    Effective concentration 1-80 µM.
    Structure
    Chemical name 2-(2-Amino-3-methoxyphenyl)-4H-1-benzopyran-4-one.
    Molecular formula C16H13NO3.
    CAS No.: 167869-21-8.
    Activity PD 98059 specifically inhibits the mitogen-activated protein kinase kinase (MAPKK) also known as MEK1 and MEK2. It is commonly used in studies of the MAPK pathway.
    References-Activity
    1. Dudley, D.T. et al. (1995) Proc. Natl. Acad. Sci. U.S.A. 92, 7686.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
    Storage of solutions Up to two weeks at 4°C or six months at -20°C.
    Protect from light.
    Our bioassay
    • Alomone Labs PD 98059 inhibits P42/44 MAPK (ERK) phosphorylation via MEK in C6 glioma cells.
      Alomone Labs PD 98059 inhibits P42/44 MAPK (ERK) phosphorylation via MEK in C6 glioma cells.
      Cells were grown to 70% confluence and serum starved for 1.5 h. The cells were then incubated for 2 h with various concentrations of PD 98059 (#P-260) and stimulated with 7 ng/ml PDGF-AA. Cell proteins were resolved by SDS-PAGE and probed with anti-phospho-P42/44 (upper panel) and with anti-P42/44 (lower panel) antibodies. Inhibition of P42/44 phosphorylation by PD 98059 was dose-dependent.
    References - Scientific background
    1. Alessi, D.R. et al. (1995) J. Biol. Chem. 270, 27489.
    2. Dudley, D.T. et al. (1995) Proc. Natl. Acad. Sci. 92, 7686.
    3. Pang, L. et al. (1995) J. Biol. Chem. 270, 13585.
    4. Yamaguchi, K. et al. (2002) J. Ophthalmol. 46, 634.
    5. Sjogreen, B. et al. (2000) Neuroscience 100, 407.
    6. Coogan, A.N. et al. (1999) J. Neurophysiol81, 103.
    7. English, J. D. et al. (1997) J. Biol. Chem. 272, 19103.
    8. Guyton, K.Z. et al. (1996) Cancer Res. 56, 3480.
    9. Cook, S.J. et al. (1997) J. Biol. Chem.  272, 13309.
    10. Reiners, J.J. Jr. et al. (1998) Mol. Pharmacol53, 438.
    Scientific background

    PD 98059 is a synthetic flavonoid and a potent inhibitor of mitogen-activated protein kinase (MAPK) by inhibiting its immediate upstream activator, mitogen activated protein kinase kinase (also known as MEK or MKK1).1-3

    PD 98059 arrests cell cycle progression of some rat cultured cells at the G0/G1 phase and blocks nerve growth factor stimulated axonal outgrowth from adult mouse dorsal root ganglia in vitro,4-5 and blocks induction of LTP in the brain areas.6-7

    PD 98059 is also an equipotent antagonist of aryl hydrocarbon receptor (AHR) stimulated by TCDD in MCF10A cells. 8-10

    Last update: 16/08/2020

    PD 98059 (#P-260) is a highly pure, synthetic, and biologically active compound.

    For research purposes only, not for human use

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    Scientific Background

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