PEX5L/TRIP8b Blocking Peptide (#BLP-PR070) is the original antigen used for immunization during Anti-PEX5L (TRIP8b) Antibody (#APR-070) generation. The blocking peptide binds and ‘blocks’ Anti-PEX5L/TRIP8b primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:
- Expression of PEX5-related protein in rat brainImmunohistochemical staining of rat parietal cortex using Anti-PEX5L (TRIP8b) Antibody (#APR-070), (1:300). A. PEX5Rp (green) is labeled in dendritic and axonal profiles (arrows). B. Nuclear staining using DAPI as the counterstain. C. Merged image of A and B.
- (C)EKWDDVKFHGDRTSK, corresponding to amino acid residues 151-165 of rat PEX5-related protein (Accession Q925N3).