Piezo2 Blocking Peptide (#BLP-PC090) is the original antigen used for immunization during Anti-Piezo2 Antibody (#APC-090) generation. The blocking peptide binds and ‘blocks’ Anti-Piezo2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat dorsal root ganglion lysate (lanes 1 and 3) and mouse lung lysate (lanes 2 and 4):
- Western blot analysis of human HepG2 hepatocellular cell line lysate (lanes 1 and 3) and human HUVEC endothelial cell line lysate (lanes 2 and 4):
- Expression of Piezo2 in rat dorsal root ganglion.Immunohistochemical staining of perfusion-fixed frozen rat dorsal root ganglion (DRG) sections with Anti-Piezo2 Antibody (#APC-090), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Piezo2 immunoreactivity (green) appeared in neuron profiles (arrows). B. Pre-incubation of the antibody with Piezo2 Blocking Peptide (#BLP-PC090), suppressed staining. Cell nuclei are stained with DAPI (blue).
- Peptide (C)RTIFHDITRLHLD, corresponding to amino acid residues 1092 - 1104 of human Piezo 2 (Accession Q9H5I5). The peptide is located in an internal region of human Piezo2 (the exact topology of Piezo2 remains to be defined).