Overview
Type: Synthetic peptide
Form: Lyophilized Powder
Piezo2 Blocking Peptide (#BLP-PC090) is the original antigen used for immunization during Anti-Piezo2 Antibody (#APC-090) generation. The blocking peptide binds and ‘blocks’ Anti-Piezo2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat dorsal root ganglion lysate (lanes 1 and 3) and mouse lung lysate (lanes 2 and 4):1, 2. Anti-Piezo2 Antibody (#APC-090), (1:200).
3, 4. Anti-Piezo2 Antibody, preincubated with Piezo2 Blocking Peptide (#BLP-PC090).
Western blot analysis of human HepG2 hepatocellular cell line lysate (lanes 1 and 3) and human HUVEC endothelial cell line lysate (lanes 2 and 4):1, 2. Anti-Piezo2 Antibody (#APC-090), (1:200).
3, 4. Anti-Piezo2 Antibody, preincubated with Piezo2 Blocking Peptide (#BLP-PC090).
Expression of Piezo2 in rat dorsal root ganglion.Immunohistochemical staining of perfusion-fixed frozen rat dorsal root ganglion (DRG) sections with Anti-Piezo2 Antibody (#APC-090), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Piezo2 immunoreactivity (green) appeared in neuron profiles (arrows). B. Pre-incubation of the antibody with Piezo2 Blocking Peptide (#BLP-PC090), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- Peptide (C)RTIFHDITRLHLD, corresponding to amino acid residues 1092 - 1104 of human Piezo 2 (Accession Q9H5I5). The peptide is located in an internal region of human Piezo2 (the exact topology of Piezo2 remains to be defined).
Accession (Uniprot) Number Q9H5I5
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
