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PMA

Phorbol 12-myristate 13-acetate, TPA, 12-O-tetradecanoylphorbol-13-acetate
A PKC Activator
Cat #: P-800
Alternative Name Phorbol 12-myristate 13-acetate, TPA, 12-O-tetradecanoylphorbol-13-acetate
Lyophilized Powder yes
  • Bioassay Tested
  • Origin Croton plant.
    Source Semi-synthetic
    MW: 616.84
    Purity: >99.5%
    Effective concentration 200 nM.
    Structure
    Chemical name (1aR,1bS,4aR,7aS,7bS,8R,9R,9aS)-9a-(Acetyloxy)- 1a,1b,4, 4a,5,7a,7b,8,9,9a-decahydro-4a,7b-dihydroxy-3-(hydroxymethyl)-1,1,6,8-tetramethyl-5-oxo-1H-cyclopropa[3,4]benz [1,2-e]azulen-9-yl tetradecanoate.
    Molecular formula C36H56O8.
    CAS No.: 16561-29-8.
    Activity PMA is a commonly used phorbol ester and activates PKC in nM concentrations1. It has also been shown to be an extremely potent tumor promoter2-4.
    References-Activity
    1. Murray, A.W. and Froscio, M. (1977) Cancer Res37, 1360.
    2. Janoff, A. et al. (1970) Cancer Res. 10, 2568.
    3. Weinstein, I.B. (1985) Carcinog. Compr. Surv10, 177.
    4. Isakov, N. et al. (1985) Biochem. Biophys. Res. Commun31, 724.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility DMSO or methanol. Centrifuge all product preparations before use (10000 x g 5 min).
    Storage of solutions Up to four weeks at 4°C or three months at -20°C.
    Highly irritable to skin and mucous membranes. Tumor promoter. Gloves and mask should be worn when using this product.
    Our bioassay
    • Alomone Labs PMA activates PKC in NIH-3T3 cells.
      Alomone Labs PMA activates PKC in NIH-3T3 cells.
      Cells were stimulated with 0.6 µM PMA (#P-800) for 20 min. Cell proteins were resolved by SDS-PAGE and probed with anti-phospho (ser)- MARCKS (myristoylated alanin-rich protein kinase C substrate).
    References - Scientific background
    1. Janoff, A. et al. (1970) Cancer Res10, 2568.
    2. Weinstein, I.B. (1985) Carcinog. Compr. Surv10, 177.
    3. Isakov, N. et al. (1985) Biochem. Biophys. Res. Commun31, 724.
    4. Murray, A.W. and Froscio, M. (1977) Cancer Res37, 1360.
    5. Tepper, C.G. et al. (1995) Proc. Natl. Acad. Sci. USA 92, 8443.
    6. Oishi, K. et al. (1994) J. Cell. Biochem. 55, 168.
    7. Macfarlane, D.E. and O'Donnell, P.S. (1993) Leukemia 7, 1846.
    8. Hortelano, S. et al. (1992) J. Biol. Chem267, 24937.
    Scientific background Phorbol 12-myristate 13-acetate (PMA) is the most commonly-used phorbol ester, primary found as extremely potent tumor promoter.1-3 PMA is a reversible activator of Protein Kinase C (PKC)4 in vivo and in vitro, even at nM concentrations (cells are usually treated with PMA at 200 nM to induce signaling). PMA promotes the expression of inducible NOS in cultured hepatocytes, activates Ca2+-ATPase pump and potentates forskolin-induced cAMP formation. In some cases PMA inhibits apoptosis induced by the Fas antigen, but induces apoptosis in HL-60 promyelocytic leukemia cells.5-8
    Last update: 16/08/2020

    PMA (#P-800) is a highly pure, semi-synthetic, and biologically active compound.

    For research purposes only, not for human use

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    Scientific Background

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