Overview
Type: Synthetic peptide
Form: Lyophilized powder
proGDNF Blocking Peptide (#BLP-NT026) is the original antigen used for immunization during Anti-proGDNF Antibody (#ANT-026) generation. The blocking peptide binds and ‘blocks’ Anti-proGDNF primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of human GDNF pro-domain fusion protein at 40 ng (lanes 1 and 4), 30 ng (lanes 2 and 5) and 20ng (lanes 3 and 6):1-3. Anti-proGDNF Antibody (#ANT-026), (1:200).
4-6. Anti-proGDNF Antibody, preincubated with proGDNF Blocking Peptide (#BLP-NT026).
Note that the expected MW of the GDNF pro-domain fusion protein is 21kD.
Properties
Sequence
- (C)PEDYPDQFDDVMD, corresponding to amino acid residues 54-66 of human GDNF (precursor) (Accession P39905).
Accession (Uniprot) Number P39905
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
