RIC3 Blocking Peptide (#BLP-NC020) is the original antigen used for immunization during Anti-RIC3 Antibody (#ANC-020) generation. The blocking peptide binds and ‘blocks’ Anti-RIC3 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate:
- Western blot analysis of mouse heart membranes (lanes 1 and 4), rat heart lysate (lanes 2 and 5) and rat brain lysate (lanes 3 and 6):
- Expression of RIC-3 in mouse olfactory bulb and fornixImmunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-RIC3 Antibody (#ANC-020), (1:400), followed by goat-anti-rabbit-AlexaFluor-488. A. RIC-3 staining in mouse olfactory bulb shows staining in the external plexiform layer (EPL) and in some glomeruli (G). Arrows point at glomeruli with RIC-3 positive core. B. RIC-3 staining in mouse fornix is detected in neuronal profiles (vertical arrows) and neuronal processes (horizontal arrow). Cell nuclei are stained with DAPI (blue).
- (C)EKLINRVGPNGESR, corresponding to amino acid residues 161-174 of mouse RIC3 (Accession Q8BPM6).