SAP102 Blocking Peptide (#BLP-PZ003) is the original antigen used for immunization during Anti-SAP102 Antibody (#APZ-003) generation. The blocking peptide binds and ‘blocks’ Anti-SAP102 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain membranes:1. Anti-SAP102 Antibody (#APZ-003), (1:400).
2. Anti-SAP102 Antibody, preincubated with the control fusion protein antigen.
- Rat brain sections.
- GST fusion protein with the sequence KSTPKLNGSGPSWWPECTCTNRDWYEQVNGSD, corresponding to amino acid residues 93-124 of human SAP102 (Accession Q92796).