SCN1A/Nav1.1 Blocking Peptide (#BLP-SC001) is the original antigen used for immunization during Anti-SCN1A (NaV1.1) Antibody (#ASC-001) generation. The blocking peptide binds and ‘blocks’ Anti-SCN1A/Nav1.1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain membranes:
- Expression of NaV1.1 sodium channel in mouse cerebellumImmunohistochemical staining of mouse cerebellum using Anti-SCN1A (NaV1.1) Antibody (#ASC-001). A. The distribution of NaV1.1 (red) forms a band (arrows) in the molecular layer (Mol), close to the Purkinje cell bodies. B. Purkinje nerve cells are stained with mouse anti-Parvalbumin (green). C. Confocal merge of NaV1.1 and Parvalbumin.
- Human brain (1:400) (Wang, W. et al. (2011) Brain Res. 1389, 61.).
- Western blot analysis of rat brain (lanes 1 and 3) and mouse brain (lanes 2 and 4) lysates:
- (C)TASEHSREPSAAGRLSD, corresponding to amino acid residues 465-481 of rat NaV1.1 (Accession P04774).