Overview
Type: Synthetic peptide
Form: Lyophilized powder
SCN1B/Navβ1 (extracellular) Blocking Peptide (#BLP-SC041) is the original antigen used for immunization during Anti-SCN1B (NaVβ1) (extracellular) Antibody (#ASC-041) generation. The blocking peptide binds and ‘blocks’ Anti-SCN1B/Navβ1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only, not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) and rat skeletal muscle (lanes 3 and 6):1-3. Anti-SCN1B (NaVβ1) (extracellular) Antibody (#ASC-041), (1:400).
4-6. Anti-SCN1B (NaVβ1) (extracellular) Antibody, preincubated with SCN1B/Navβ1 (extracellular) Blocking Peptide (#BLP-SC041).
Expression of NaVβ1 in rat DRGImmunohistochemical staining of adult rat dorsal root ganglion (DRG) using Anti-SCN1B (NaVβ1) (extracellular) Antibody (#ASC-041) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. A. NaVβ1 labeling (red) appears in the cell bodies of the DRG neurons. B. Nuclear staining using DAPI as the counterstain (blue). C. Merged image of A and B.
Properties
Sequence
- CKRRSETTAETFTE, corresponding to amino acid residues 43-56 of rat β1 subunit of voltage-gated Na+ channels (Accession Q00954).
Accession (Uniprot) Number Q00954
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
