Anti-NaVβ1 (extracellular) Antibody

Sodium channel subunit beta-1
    Cat #: ASC-041
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: m, r
    May also work in: h
    Immunogen
    Peptide CKRRSETTAETFTE, corresponding to amino acid residues 43-56 of rat Navβ1 (Accession Q00954). Extracellular, N-terminus.
    Accession (Uniprot) Number Q00954
    Gene ID 29686
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - identical; human -13/14 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.85 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20ºC.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, ih, lci, wb
    May also work in: ifc, ip
    Western blot
    Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) and rat skeletal muscle (lanes 3 and 6):
    1-3. Anti-NaVβ1 (extracellular) Antibody (#ASC-041), (1:400).
    4-6. Anti-NaVβ1 (extracellular) Antibody, preincubated with the control peptide antigen.
    Immunohistochemistry
    Expression of NaVβ1 in rat DRG
    Immunohistochemical staining of adult rat dorsal root ganglion (DRG) using Anti- NaVβ1 (extracellular) Antibody (#ASC-041) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. A. Navβ1 labeling (red) appears in the cell bodies of the DRG neurons. B. Nuclear staining using DAPI as the counterstain (blue). C. Merged image of A and B.
    Live cell imaging / Immunocytochemistry
    Expression of NaVβ1 in differentiated rat PC12 cells
    Immunocytochemical staining of live and intact differentiated rat PC12 cells. PC12 differentiation was induced by Native mouse NGF 2.5S protein (>95%) (#N-100). A, C. Merge of NaVβ1 staining using Anti-NaVβ1 (extracellular) Antibody (#ASC-041), (1:50), (red) with live cell imaging.  B, D. Merge of NaVβ1 staining (1:50), (red) with nuclear staining using DAPI as the counterstain.
    References
    1. Wu, L. et al. (2002) NeuroReport 13, 2547.
    2. Baker, M.D. and Wood, J.N. (2001) Trends Pharmacol. Sci. 22, 27.
    3. Lai, J. et al. (2003) Curr.Opin. Neurobiol 13, 291.
    4. Catterall, W.A. (2000) Neuron 26, 13.
    5. Chen, C. et al. (2002) Proc. Natl. Acad. Sci. U.S.A. 99, 17072.
    6. Schmidt, J.W. and Catterall, W.A. (1986) Cell 46, 437.
    7. Lopez-Santiago L. F. et al. (2006) J. Neurosci. 6, 7984.
    Scientific background

    Voltage-gated sodium channels (Nav) are essential for the generation of action potentials and for cell excitability.1 To date, nine Nav α subunits have been cloned and named Nav1.1-Nav1.9.2,3  Mammalian sodium channels are heterotrimers, composed of a central, pore-forming α subunit and two auxiliary β-subunits (Naβ-subunit).4

    The Naβ-subunit gene family consists of four members: β1 (SCN1B), β2 (SCN2B), β3 (SCN3B), and β4 (SCN4B) having type I topology, containing an extracellular amino-terminus, a single transmembrane segment, and an intracellular carboxyl-terminus.They modulate channel gating, assembly, and cell surface expression in heterologous cell systems.5

    Navβ-subunits are cell adhesion molecules of the Ig superfamily, which interact with extracellular matrix, transmembrane signaling, and cell adhesion molecules.5

    In the adult central nervous system and heart, sodium channels are associate with β1- β4 subunits, whereas in adult skeletal muscle they are associate only with the β1 subunit.4,5
    This association appears to be a late event in sodium channel biosynthesis.6

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 30/10/2018

    Anti-NaVβ1 (extracellular) Antibody (#ASC-041) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry, and live cell imaging applications. It has been designed to recognize NaVβ1 from human, rat, and mouse samples.

    For research purposes only, not for human use