Septin-7 Blocking Peptide (#BLP-IP047) is the original antigen used for immunization during Anti-Septin-7 Antibody (#AIP-047) generation. The blocking peptide binds and ‘blocks’ Anti-Septin-7 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat spleen (lanes 1 and 4), mouse spleen (lanes 2 and 5) and mouse brain (lanes 3 and 6) lysates:
- Western blot analysis of human Burkitt's lymphoma Daudi (lanes 1 and 3), human T-cell leukemia Jurkat (lanes 2 and 5) and human breast adenocarcinoma MCF-7 cell lysates (lanes 3 and 6):
- (C)HRIKKTVQVEQSK, corresponding to amino acid residues 83-96 of rat Septin-7 (Accession Q9WVC0).