SERCA3 Blocking Peptide (#BLP-CP014) is the original antigen used for immunization during Anti-SERCA3 Antibody (#ACP-014) generation. The blocking peptide binds and ‘blocks’ Anti-SERCA3 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes:
- Expression of SERCA3 in rat cerebellumImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-SERCA3 Antibody (#ACP-014), (1:200), followed by goat anti-rabbit-AlexaFluor-488. SERCA3 immunoreactivity (green) appears in the molecular layer (MOL) and Purkinje cells (arrows) but not in the granule layer (GL) or cerebellum sections. Cell nuclei are stained with DAPI (blue).
- (C)DEALKYLSRHHVD, corresponding to amino acid residues 981-993 of rat SERCA3 (Accession P18596).