Overview
Type: Synthetic peptide
Form: Lyophilized powder
SGLT1 (extracellular) Blocking Peptide (#BLP-GT031) is the original antigen used for immunization during Anti-SGLT1 (extracellular) Antibody (#AGT-031) generation. The blocking peptide binds and ‘blocks’ Anti-SGLT1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:1,2. Anti-SGLT1 (extracellular) Antibody (#AGT-031), (1:200).
3,4. Anti-SGLT1 (extracellular) Antibody, preincubated with SGLT1 (extracellular) Blocking Peptide (#BLP-GT031).
Western blot analysis of rat small intestine lysate:1. Anti-SGLT1 (extracellular) Antibody (#AGT-031), (1:200).
2. Anti-SGLT1 (extracellular) Antibody, preincubated with SGLT1 (extracellular) Blocking Peptide (#BLP-GT031).
Expression of SGLT1 in rat brain sectionsImmunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-SGLT1 (extracellular) Antibody (#AGT-031), (1:400). A. SGLT1 staining in the hippocampal dentate gyrus reveals labeling of cells (red) in the granule layer (vertical arrows) and in hilar neurons (horizontal arrow). B. SGLT1 staining in the rat hypothalamic paraventricular nucleus region (in red), appears in cells lining the wall of the third ventricle (horizontal arrows) and in neurons of the paraventricular nucleus (vertical arrows). Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)TDAPIQSYERIRN, corresponding to amino acid residues 13-25 of rat SGLT1 (Accession P5379).
Accession (Uniprot) Number P5379
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
