SGLT1 (extracellular) Blocking Peptide (#BLP-GT031) is the original antigen used for immunization during Anti-SGLT1 (extracellular) Antibody (#AGT-031) generation. The blocking peptide binds and ‘blocks’ Anti-SGLT1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:
- Western blot analysis of rat small intestine lysate:
- Expression of SGLT1 in rat brain sectionsImmunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-SGLT1 (extracellular) Antibody (#AGT-031), (1:400). A. SGLT1 staining in the hippocampal dentate gyrus reveals labeling of cells (red) in the granule layer (vertical arrows) and in hilar neurons (horizontal arrow). B. SGLT1 staining in the rat hypothalamic paraventricular nucleus region (in red), appears in cells lining the wall of the third ventricle (horizontal arrows) and in neurons of the paraventricular nucleus (vertical arrows). Cell nuclei are stained with DAPI (blue).
- (C)TDAPIQSYERIRN, corresponding to amino acid residues 13-25 of rat SGLT1 (Accession P5379).