Shank3 Blocking Peptide (#BLP-PZ013) is the original antigen used for immunization during Anti-Shank3 Antibody (#APZ-013) generation. The blocking peptide binds and ‘blocks’ Anti-Shank3 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain synaptosomal fractions:
- Expression of Shank3 in rat and mouse parietal cortexImmunohistochemical staining of perfusion-fixed frozen rat and mouse brain sections using Anti-Shank3 Antibody (#APZ-013), (1:400), followed by anti-rabbit-Cy3 antibody (red). Shank3 staining of both rat (A) and mouse (B) brain sections, appears in neuron processes (horizontal arrows). Nuclei are stained with DAPI (blue).
- (C)EKLPGSLRKGIPRTK, corresponding to amino acid residues 841-855 of rat Shank3 (Accession Q9JLU4).