Overview
Type: Synthetic peptide
Form: Lyophilized powder
Sigma-1 Receptor Blocking Peptide (#BLP-IP006) is the original antigen used for immunization during Anti-Sigma-1 Receptor Antibody (#AIP-006) generation. The blocking peptide binds and ‘blocks’ Anti-Sigma-1 Receptor primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) liver membranes:1,2. Anti-Sigma-1 Receptor Antibody (AIP-006), (1:200).
3,4. Anti-Sigma-1 Receptor Antibody, preincubated with Sigma-1 Receptor Blocking Peptide (#BLP-IP006).
Expression of Sigma-1 Receptor in rat spinal cord.Immunohistochemical staining of perfusion-fixed frozen rat spinal cord sections with Anti-Sigma-1 Receptor Antibody (#AIP-006), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Sigma-1 Receptor immunoreactivity (green) appeared in the superficial layer of the dorsal horn (arrows). B. Pre-incubation of the antibody with Sigma-1 Receptor Blocking Peptide (BLP-IP006), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)SRGHSGRYWAEISD, corresponding to amino acid residues 113-126 of human SIGMAR1 (Accession Q99720).
Accession (Uniprot) Number Q99720
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
