Overview
Type: Synthetic peptide
Form: Lyophilized powder
SLC38A1 Blocking Peptide (#BLP-NT184) is the original antigen used for immunization during Anti-SLC38A1 Antibody (#ANT-184) generation. The blocking peptide binds and ‘blocks’ Anti-SLC38A1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysate (lanes 1 and 3), rat placenta (lanes 2 and 4), (1:800), mouse brain membrane (lanes 5 and 6), (1:200), human U-87 MG (lanes 7 and 9), and human Jurkat (lanes 8 and 10), (1:500) cell lysates:1, 2, 5, 7, 8. Anti-SLC38A1 Antibody (#ANT-184).
3, 4, 6, 9, 10. Anti-SLC38A1 Antibody, preincubated with SLC38A1 Blocking Peptide (#BLP-NT184).
Expression of SLC38A1 in rat cerebellum.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-SLC38A1 Antibody (#ANT-184), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. SLC38A1 immunoreactivity (green) appears in neurons of the purkinje cell layer (P, vertical arrows) and in dendrites in the molecular layer (MOL, horizontal arrows). B. Pre-incubation of the antibody with SLC38A1 Blocking Peptide (BLP-NT184), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)SKFISDRESRRSLTNSH, corresponding to amino acid residues 41-57 of rat SLC38A1 (Accession Q9JM15).
Accession (Uniprot) Number Q9JM15
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
