SLC38A1 Blocking Peptide (#BLP-NT184) is the original antigen used for immunization during Anti-SLC38A1 Antibody (#ANT-184) generation. The blocking peptide binds and ‘blocks’ Anti-SLC38A1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain lysate (lanes 1 and 3), rat placenta (lanes 2 and 4), (1:800), mouse brain membrane (lanes 5 and 6), (1:200), human U-87 MG (lanes 7 and 9), and human Jurkat (lanes 8 and 10), (1:500) cell lysates:
- Expression of SLC38A1 in rat cerebellum.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-SLC38A1 Antibody (#ANT-184), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. SLC38A1 immunoreactivity (green) appears in neurons of the purkinje cell layer (P, vertical arrows) and in dendrites in the molecular layer (MOL, horizontal arrows). B. Pre-incubation of the antibody with SLC38A1 Blocking Peptide (BLP-NT184), suppressed staining. Cell nuclei are stained with DAPI (blue).
- (C)SKFISDRESRRSLTNSH, corresponding to amino acid residues 41-57 of rat SLC38A1 (Accession Q9JM15).