Overview
Type: Synthetic peptide
Form: Lyophilized Powder
SLC38A2 (SNAT2) Blocking Peptide (#BLP-NT185) is the original antigen used for immunization during Anti-SLC38A2 (SNAT2) Antibody (#ANT-185) generation. The blocking peptide binds and ‘blocks’ Anti-SLC38A2 (SNAT2) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):1-2. Anti-SLC38A2 (SNAT2) Antibody (#ANT-185), (1:400).
3-4. Anti-SLC38A2 (SNAT2) Antibody, preincubated with SLC38A2 (SNAT2) Blocking Peptide (BLP-NT185).
Western blot analysis of human HepG2 hepatocellular carcinoma cell line lysate (lanes 1 and 4), human LNCaP prostate adenocarcinoma cell line lysate (lanes 2 and 5) and human Jurkat T-cell lymphoma cell line lysate (lanes 3 and 6):1-3. Anti-SLC38A2 (SNAT2) Antibody (#ANT-185), (1:400).
4-6. Anti-SLC38A2 (SNAT2) Antibody, preincubated with SLC38A2 (SNAT2) Blocking Peptide (BLP-NT185).
Expression of SLC38A2 in rat hypothalamus.Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-SLC38A2 (SNAT2) Antibody (#ANT-185), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. SLC38A2 immunoreactivity (green) appears in neuronal outlines (horizontal arrows) in the ventromedial hypothalamus and in cells lining the wall of the 3rd ventricle (vertical arrows). B. Pre-incubation of the antibody with SLC38A2 (SNAT2) Blocking Peptide (BLP-NT185), suppressed staining. Cell nuclei are stained with DAPI (blue). 3rd V = third ventricle.
Expression of SLC38A2 in rat spinal cord.Immunohistochemical staining of perfusion-fixed frozen rat spinal cord sections using Anti-SLC38A2 (SNAT2) Antibody (#ANT-185), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the ventral horn, showed SLC38A2 immunoreactivity (green), in neuronal outlines (arrows). B. Pre-incubation of the antibody with SLC38A2 (SNAT2) Blocking Peptide (BLP-NT185), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- Peptide (C)DFNYSYPTKQAALKSH, corresponding to amino acid residues 25 - 40 of mouse SLC38A2 (Accession Q8CFE6).
Accession (Uniprot) Number Q8CFE6
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
