SLC6A8 Blocking Peptide (#BLP-GT008) is the original antigen used for immunization during Anti-SLC6A8 Antibody (#AGT-008) generation. The blocking peptide binds and ‘blocks’ Anti-SLC6A8 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of mouse heart (lanes 1 and 4), mouse brain (lanes 2 and 5) and rat brain (lanes 3 and 6) lysates:
- Western blot analysis of human HepG2 liver hepatocellular carcinoma:
- Expression of creatine transporter in rat hippocampus and cortexImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-SLC6A8 Antibody (#AGT-008), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Creatine transporter staining (green) in the hippocampal CA1 region is detected in apical dendrites (arrows). B. Creatine transporter immunoreactivity (green) in cortex is observed in soma and apical dendrites of neurons (arrows). Cell nuclei are stained with DAPI (blue).
- (C)RLAVPPRETWTRQMD, corresponding to amino acid residues 46-60 of rat creatine transporter (Accession P28570).