SMCT1 (SLC5A8) Blocking Peptide (#BLP-GT036) is the original antigen used for immunization during Anti-SMCT1 (SLC5A8) Antibody (#AGT-036) generation. The blocking peptide binds and ‘blocks’ Anti-SMCT1 (SLC5A8) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of mouse brain lysate (lanes 1 and 4), rat kidney membranes (lanes 2 and 5) and mouse colon membranes (3 and 6):
- Western blot analysis of human LoVo colorectal adenocarcinoma cell line lysate (lanes 1 and 3) and human ARPE-19 retinal pigmented epithelium cell line lysate (2 and 4):
- Expression of SMCT1 in rat supraoptic nucleus.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-SMCT1 (SLC5A8) Antibody (#AGT-036), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. SMCT1 immunoreactivity (green) was detected in neuronal profiles (arrows). B. Pre-incubation of the antibody with SMCT1 (SLC5A8) Blocking Peptide (BLP-GT036), suppressed staining. Cell nuclei are stained with DAPI (blue).
- Peptide (C)GGRKQNLDPRYILTK, corresponding to amino acid residues 544 - 558 of human SMCT1 (Accession Q8N695).