SVCT2/SLC23A2 (extracellular) Blocking Peptide (#BLP-ST022) is the original antigen used for immunization during Anti-SVCT2/SLC23A2 (extracellular) Antibody (#AST-022) generation. The blocking peptide binds and ‘blocks’ Anti-SVCT2/SLC23A2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:
- Western blot analysis of human MCF-7 breast adenocarcinoma (lanes 1 and 3) and human COLO 205 colon adenocarcinoma (lanes 2 and 4) cell line lysates:
- Expression of SVCT2 in mouse hippocampusImmunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-SVCT2/SLC23A2 (extracellular) Antibody (#AST-022), (1:200), followed by goat anti-rabbit-AlexaFluor-488. SVCT2 staining (green) in CA1 hippocampal region is detected in axonal processes (arrows), with the highest density surrounding the pyramidal layer (P). Cell nuclei are stained with DAPI (blue).
- (C)EITVANGTAELLEH, corresponding to amino acid residues 191-204 of mouse SVCT2 (Accession Q9EPR4).