Overview
Type: Synthetic peptide
Form: Lyophilized powder
Syntaxin 4 Blocking Peptide (#BLP-NR004) is the original antigen used for immunization during Anti-Syntaxin 4 Antibody (#ANR-004) generation. The blocking peptide binds and ‘blocks’ Anti-Syntaxin 4 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot anlysis of rat heart lysate:1. Anti-Syntaxin 4 Antibody (ANR-004), (1:400).
2. Anti-Syntaxin 4 Antibody, preincubated with Syntaxin 4 Blocking Peptide (#BLP-NR004).
Expression of syntaxin 4 in rat pancreasImmunohistochemical staining of rat pancreas paraffin embedded sections using Anti-Syntaxin 4 Antibody (#ANR-004), (1:100). STX4 (brown staining) is expressed in endocrine cells of the Isles of Langerhans (arrow). Hematoxilin is used as the counterstain.
Properties
Sequence
- RDRTHELRQGDNISDDEDEVRV(C), corresponding to residues 2-23 of rat syntaxin 4 (Accession Q08850).
Accession (Uniprot) Number Q08850
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
