Syntaxin 4 Blocking Peptide (#BLP-NR004) is the original antigen used for immunization during Anti-Syntaxin 4 Antibody (#ANR-004) generation. The blocking peptide binds and ‘blocks’ Anti-Syntaxin 4 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot anlysis of rat heart lysate:
- Expression of syntaxin 4 in rat pancreasImmunohistochemical staining of rat pancreas paraffin embedded sections using Anti-Syntaxin 4 Antibody (#ANR-004), (1:100). STX4 (brown staining) is expressed in endocrine cells of the Isles of Langerhans (arrow). Hematoxilin is used as the counterstain.
- RDRTHELRQGDNISDDEDEVRV(C), corresponding to residues 2-23 of rat syntaxin 4 (Accession Q08850).