TGR5/GPBAR1 Blocking Peptide (#BLP-BR031) is the original antigen used for immunization during Anti-TGR5 (GPBAR1) Antibody (#ABR-031) generation. The blocking peptide binds and ‘blocks’ Anti-TGR5/GPBAR1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain (lanes 1 and 4), mouse liver (lanes 2 and 5) and rat spleen (lanes 3 and 6) lysates:
- (C)GDQRYTAPWRTAAQR, corresponding to amino acid residues 282-296 of rat GPBAR1 (Accession Q80T02).