Overview
Type: Synthetic peptide
Form: Lyophilized powder
TMEM66 Blocking Peptide (#BLP-CC067) is the original antigen used for immunization during Anti-TMEM66 Antibody (#ACC-067) generation. The blocking peptide binds and ‘blocks’ Anti-TMEM66 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only, not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat pancreas (lanes 1 and 2), Mouse MS1 endothelial cells (lanes 3 and 5) and human PANC1 pancreatic carcinoma cells (lanes 4 and 6) lysates:1,3,4. Anti-TMEM66 Antibody (#ACC-067), (1:500).
2,5,6. Anti-TMEM66 Antibody, preincubated with TMEM66 Blocking Peptide (#BLP-CC067).
Expression of TMEM66 in mouse cerebellumImmunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-TMEM66 Antibody (#ACC-067), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. TMEM66 immunoreactivity (green) appears in the molecular layer (MOL) and in neurons of the purkinje cell layer (P, arrows). B. Pre-incubation of the antibody with TMEM66 Blocking Peptide (#BLP-CC067), suppresses staining. Cell nuclei are stained with DAPI (blue). G = granule layer.- Expression of TMEM66 in mouse heartImmunohistochemical staining of frozen mouse heart sections with Anti-TMEM66 Antibody (#ACC-067), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. TMEM66 staining (green) appears along the lateral wall of muscle fibers (arrows). B. Pre-incubation of the antibody with TMEM66 Blocking Peptide (#BLP-CC067), suppresses staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)ELGLSKLKESGKHQS, corresponding to amino acid residues 141-155 of rat SARAF (Accession Q6AYN2).
Accession (Uniprot) Number Q6AYN2
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
